Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKEEIIERFTTYVKVDTQSDESVDTCPSTPGQLTLGNMLVDELKSIGMQDAAID-ENGYVMATLPSNTEKDVPTIGFLAHVDTATDFTGKNVNPQIIESYDGKDIVLNEQLQVTLSPDQFPELSGYKGHTLITTDGTTLLGADNKAGIAEIMTAMDYLIKHPEIKHGTIRVAFTPDEEIGR-GPHKFDVKR-F-NASFAYTVDGGP---LG----ELEYESFNAAAAKITIKGNNVHPGTAKGKMINSAKIAMKLNSLLPADEAPEYTEGYEGFYHLLSIQGD------VEETKLHYIIRDFDKENFQNRKETMKRAVEELQNEYGQDRILLDMNDQYYNMREKIEPVIEIVNIAKQAMENLGIEP---KISPIRGGTDGSQLSYMGLPTPNI-FTGG-----ENFHGKFEYISVDNMVKAVNVIVEIAKQFEAQA 4EWT Chain:A ((13-389)) -EGKMIEIRRYLHQHPELSFHE----------DETAKYIAEFYKGKDVE-VETNVGPRGIKVTIDSG--KPGKTLAIRADFDALPITEDTGL-S-----FAS--Q----------------------NKGVMHACG----H-D--AHTAYMLVLAETLAEMKDSFTGKVVVIHQPAEEVPPGGAKTMIENGVLDGVDHVLGVHVMSTMKTGKVYYRPGYVQTGRAFFKLKVQGKGGHGSSP-HMANDAIVAGSYFVTALQTVVSRRLSPFETGVVTIGSFDGKGQFNVIKDVVEIEGDVRGLTDATKATIEKEIKRLSKGLEDMYG-VTCTLEYNDDYPAL--Y--NDPEFTEYVAKTLKEANLDFGVEMCEPQPPSEDFAYYAKERPSAFIYTGAAVENGEIYPHHHPKFNISEKSLLISAEAVGTVVLDYLK--

General information: TITO was launched using: RESULT: Template: 4EWT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1874 58620 31.28 167.01 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 31.28 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.496

(partial model without unconserved sides chains): PDB file : Tito_4EWT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EWT-query.scw PDB file : Tito_Scwrl_4EWT.pdb: