Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVRFLQISDLHFQFQNY------DTIVMRDKLLDFIEVLRRESDFDFLLLTGDIAHKGDVYNE---DVKEYLNGII--------KSMG--------------------LSKNNVHLVPGNHDISRDMTRTLLIDSIMKSPNPSEMLDKLDQKATNILVEGQRKFFDFYEDFM-GVKYPEEDLHFLYQSEKYNVLSINTCLLSDKKGEEGTLLTAQMKLYKAIRKMNKEKNKGKVLNIAIGHHTLGCIESSERESIK-AHFDDYFIDLYLAGHVHDSSFNITANTNENPFLELVSGAIIKD-----EYATPEFISVDVNLDNGETEVTYYIWNTEYKYWSKNNQGGRRLQEGKLNYKINRLESLIEKEIEDNDDKIDEDEFKSFIIDFHEYRESYKTFTSNFDNQIGLDKKFYDMKSGETFKRKFDSYSEYFGVINHIMDSTSYVSADKKELIAETIVDKYLEFHNQYNNGDEIFVKIVNEIYLECHSVLPYSKALTKKYIKILTCWCIYECEIFNDNKRSVKND 4YKE Chain:A ((9-290)) -TIRILVSTDNHVGYEERDPIRKDDSWRTFDEIMQLART----KDVDMVLLGGDLFHDNKPSRKAMYQVMRSLRKNCLGMKPCELEFLSDPAEVFEGAFPHVNYYDPDINVSIPVFSIHGNHDDPSGDGHLC-S--L-----------DLLQV------AGLVN---YFGRVPEADNIHVKPILLQKGKTKLALYGMSNVRDER---IHR--TFRDNKVRFYRP-------TGDWFNLLTLHQNHYAHTPT--GYLSENML-PDFLDLVIWGHEHECLIDP-KKNPETGFHVMQPGSSIATSLVPGEAVPKHIAILSITGK------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4YKE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1188 40174 33.82 172.42 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 33.82 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.363

(partial model without unconserved sides chains): PDB file : Tito_4YKE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YKE-query.scw PDB file : Tito_Scwrl_4YKE.pdb: