Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIQDSMQFAAVESGLRFYQAYDQSLSLWPIESEAFYVSTRFGKTHIIASGPKDAPSLILLHGGLFSSAMWYPNIAAWSSQFRTYAVDIIGDKNKSIPSAAMETRADFAEWMKDVFDSLGLETAHLAGLSLGGSHIVNFLLRAPERVERAVVISPAEAFISFH-PDV-YKYAAELT-GARGAESYIK-WITGDSYDLHPLLQRQIVAGVEWQDEQRSLKPTENGFPYVFTDQELKSIQVPVLLMFGEHEAMYHQQMAFERASVLVPGIQAEIVKNAGHLLSLEQPEYVNQRVLSFLCGGIK 3OM8 Chain:A ((12-266)) --------------------------------------SDGASLAYRLDGAAEKPLLALSNSIGTTLHMWDAQLPALTRHFRVLRYDARGHGASSVPPG-PYTLARLGEDVLELLDALEVRRAHFLGLSLGGIVGQWLALHAPQRIERLVLANTSAWLGPAAQWDERIAAVLQAEDMSETAAGFLGNWFPPALLERAEPVVERFRAMLMATNRH-GLAGSFAAVRDTDLRAQLARIERPTLVIAGAYDTVTAASH-GELIAASIAGARLVTLP-AVHLSNVEFPQAFEGAVLSFLGA---

General information: TITO was launched using: RESULT: Template: 3OM8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1459 -165244 -113.26 -658.34 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -113.26 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.418

(partial model without unconserved sides chains): PDB file : Tito_3OM8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OM8-query.scw PDB file : Tito_Scwrl_3OM8.pdb: