Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYEGLEDLKVDTVNRKTLAKQVIERIVHLLSSGQLRAGDKLPTEMELMDILHVSRPVLREALSSLETLGVITRKTRGGTYFNDKIGMQPF---SVML----ALATDNLPAIIEARMALELGLVTIAAEKINEEELQRLQKTIDDIANSTDNHYGEADKEFHRIIALSANNPVVEGMIQSL-L-ITH-AKIDSQIPYRERDVTVEYHKKIYDALAKRDPYKAHYHMYEHLKFVRDKILKGMDEK 1HW1 Chain:A ((5-228)) --------------AQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVTRTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLLSVRTNISTIFIRTAFRQHPDKAQEVLATANEVAD--HADAFAELDYNIFRGLAFASGNPIYGLILNGMKGLYTRIGRH-YFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHESGEIWHRMQK--

General information: TITO was launched using: RESULT: Template: 1HW1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 811 -101347 -124.97 -473.58 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -124.97 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.466

(partial model without unconserved sides chains): PDB file : Tito_1HW1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1HW1-query.scw PDB file : Tito_Scwrl_1HW1.pdb: