Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIHLSILDQAPVSKGESPVTTLQHSVELAQLSEQWGYKRYWFAEHHSTKGLASTAPEIMIARIAAQTNTIRVGSGGVLLPQYSPFKVAETFRQLEALYPNRIDLGVGRSPGGTTKTRLALTD--GVKKSLTEFNRQLQDVSYFLTDSLP-PDHPY---AGIKAAPLI--GTAPELWVLGLGENSARRAAHQGIGYVFGHFINPER-GENAFRIYRESFRPSAHFSNPSALFTIFVICAKTDEEAEELALSQDLWLLRVGKGLD---SR-VPSIEEAKAHPYTASDKK------LIEENRKRMVIGSPTTVKQQLLDLTGCYETNEIMVLCNVFDFEAKKESYERLAELFL 1NQK Chain:A ((25-346)) -----------------RPVDHGYLQQIAQAADRLGYTGVLIPTGRS-----CEDAWLVAASMIPVTQRLKFLVA-LRPSVTSPTVAARQAATLDRLSNGRALFNLVTGSDP--QELAGDGVFLDHSERYEASAEFTQVWRRLLQRETVDFNGKHIHVRGAKLLFPAIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWG-EPPELVK-EKIEQVRAKAAAHGRK--IRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFAR----------DNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYA-ALGIDSFVLSGY--P---HLEEAYRVGELLF

General information: TITO was launched using: RESULT: Template: 1NQK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1368 -124308 -90.87 -434.64 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -90.87 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.458

(partial model without unconserved sides chains): PDB file : Tito_1NQK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1NQK-query.scw PDB file : Tito_Scwrl_1NQK.pdb: