Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTMKQFEIAAIPGDGVGKEVVAAAEKVLHTAAEVHGGLSFSFTAFPWSCDYYLEHGKMMPEDGIHTLTQFEAVFLGAVGNPKL--VPDHISLWGLLLKIRRELELSINMRPAKQMAGIT--SPLLH--PNDFDFVVIRENSEGEYSEVGGRIHRGDDEIAIQNAVFTRKATERVMRFAFELAKKRRSHVTSATKSNGIYHAMPFWDEVFQQTAADYSGIETSSQHIDALAAFFVTRPETFDVIVASNLFGDILTDISSSLMGSIGIAPSANINPSGKYPSMFEPVHGSAPDIAGQGLANPIGQIWTAKLMLDH-FGEEELGAKILDVMEQVTADGIKTRDIGGQSTTAEVTDEICSRLRKL 2Y41 Chain:B ((3-344)) ----SMKVAVLPGDGIGPEVTEAALKVLRALDEAEG-LGLAYEVFPFGGAAIDAFGEPFPEPTRKGVEEAEAVLLGSVGGPKWDGLPRKIRPETGLLSLRKSQDLFANLRPAKVFPGLERLSPLKEEIARGVDVLIVRELTGGIYF---GEPRGMSEAEAWNTERYSKPEVERVARVAFEAARKRRKHVVSVDKAN-VLEVGEFWRKTVEEVGRGYPDVALEHQYVDAMAMHLVRSPARFDVVVTGNIFGDILSDLASVLPGSLGLLPSASL---GRGTPVFEPVHGSAPDIAGKGIANPTAAILSAAMMLEHAFGLVELARKVEDAVAKALLE-TPPPDLGGSAGTEAFTATVL------

General information: TITO was launched using: RESULT: Template: 2Y41.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1998 -174479 -87.33 -520.83 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : -87.33 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.455

(partial model without unconserved sides chains): PDB file : Tito_2Y41.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Y41-query.scw PDB file : Tito_Scwrl_2Y41.pdb: