Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFIHIVGPGDSLFSIGRRYGASVDQIRGVNGLDETNIVPGQALLIPLYVYTVQPRDTLTAIAAKAFVPLERLRAANPGISPNALQAGAKITIPSISNYIAGTLSFYVLRNPDL-DRELINDYAPYSSSISIFEYHIAPNGD------------------------------IA-NQLN-D-----------------AAAIE--TTWQRRVTPLATITNLTSGGFSTEIVHQVLNNPTARTNLVNNIYDLVSTRGYGGVTIDFEQVSA----------ADRDLFTGFLRQLRDRLQAG------GYVLTIAVPAKTSDNIPWLRGYDYGGIGAVVNYMFIMAYDWHHAG-SEPGPVAPITE----------------IRRTIEFTI-AQVPSRKIIIGVPLYGYDWIIPYQPGT---------------VASAISNQNAIE-RAMRYQAPIQYSAEYQSPFFRYSDQQGRTHEVWFEDVRSMSRKMQIVREYRLQAIGAWQLTLGFTPGPWLLRKFFTIRKV 1ITX Chain:A ((9-417)) ------------------------------------------------------------------------------------------------ADSYKIVGYYPSWAAYGRNYNVADIDPTKVTHINYAFADICWNGIHGNPDPSGPNPVTWTCQNEKSQTINVPNGTIVLGDPWIDTGKTFAGDTWDQPIAGNINQLNKLKQTNPNLKTIISVGGWTW----SNRFSDVAATAATREVFANSAVDFLRKYNFDGVDLDWEYPVSGGLDGNSKRPEDKQNYTLLLSKIREKLDAAGAVDGKKYLLTIASGASAT----YAANTELAKIAAIVDWINIMTYDFNGAWQKISAHNAPLNYDPAASAAGVPDANTFNVAAGAQGHLDAGVPAAKLVLGVPFYGRGWDGCAQAGNGQYQTCTGGSSVGTWEAGSFDFYDLEANYINKNGYTRYWNDTAKVPYLYNAS---NKRFISYDDAESVGYKTAYIKSKGLGGAMFWELSGDRNK--TLQNKLKA----

General information: TITO was launched using: RESULT: Template: 1ITX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1760 -197685 -112.32 -643.92 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -112.32 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.403

(partial model without unconserved sides chains): PDB file : Tito_1ITX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ITX-query.scw PDB file : Tito_Scwrl_1ITX.pdb: