Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIISYKCPNCGSDMAFDSETGSLSCSSCGRQDNIESLP-----------KENIAAR-FSDDEAKEYQCK---NCGAVLITEAETTATTCSFCGGAAILADRLSGHLAPAKVIPFTISKQEAEQAFRKWCKKGLLTPRGFMSADRIKSITGMYIPFWMFDLNSEVQVRANCTRVHQYEEGDYICTETEHFEAFRDINLDYLKIPVDASEKMKDELMDKLEPYSYEELKDFQTAYLAGYIAEKYNYTDEELFPRAKEKISSYIDSYIHSTFSGYTSVNVRDKHIHTKN-VNSFYVLLPVWMV-SYDYERAEHIFAMNGQTGKVVGKPPISRGKVAAWFSGIAGGTFLALKLVSLMMGGGF----------------------- 4CI2 Chain:B ((71-451)) -MINFDTSLPTSHMYLGSDMEEFHGRTLHDDDSCQVIPVLPHVMVMLIPGQTLPLQLFHPQEVSMVRNLIQKDRTFAVLAYSNVREREAHFGTTAEIYAYREEQEYGIETVKVKAIGRQRFKVLEIRTQSDGIQQAKVQILPERVLPSTMSAV---QLQSLSRRHIRAFRQWWQKYQKRKFHCASLTSWPPWLYSLYDAETLMERVKRQLHEWDENLKDESLPTNPIDFSYRVAACLPIDDALRIQLLKIGSAIQRLRCELDIMNKCT--SLCCKQCQDTEITTKNEIFSLSLCGPMAAYVNPHGYIHETLTVYKACNLNLSGRPSTEH----SWFPGYAWTIAQC-RICGNHMGWKFTATKKDMSPQKFWGLTRSALLPR

General information: TITO was launched using: RESULT: Template: 4CI2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1469 -78331 -53.32 -237.37 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : -53.32 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.131

(partial model without unconserved sides chains): PDB file : Tito_4CI2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CI2-query.scw PDB file : Tito_Scwrl_4CI2.pdb: