Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNCWKTINLMKDYGAVRIILTAVCFMILVF-------ISTFLAFELLRPGTSLSDEYVSLFGGLLVVILFVHKVIHVLPIICKKRKIEKKFYILRMRTWKRIPKTTMLISLVSPFLLITPVLFYAALAFPNHAHYFCMISGIHAGYCLPDFLLALKLIKAPKTAFIDQEADGLDILVEK 1JB3 Chain:A ((6-132)) -------ELQRREEEANVVLTGTVEEIMNVDPVHHTYSCKVRVWRYLKGKDIVTHE-ILLDGGNKVVIGGFGD-----PLICDNQ----------------VSTGDTRIFFVNP---------APQYMWPAHRNELMLNSS----------LMRITLRNLEEVEHCVEEHRKLLA----

General information: TITO was launched using: RESULT: Template: 1JB3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 533 -100703 -188.94 -839.19 target 2D structure prediction score : 0.22 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -188.94 2D Compatibility (Sec. Struct. Predict.) : 0.22 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.294

(partial model without unconserved sides chains): PDB file : Tito_1JB3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JB3-query.scw PDB file : Tito_Scwrl_1JB3.pdb: