Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWRNASMNHSKRNDANDFDSMDEWLRQFFEDPFAWYDETLPIDLYETSQQYIIEADLTFLQPTQVTVTLSGCEFILTVKS---------SGQTFEKQMMLPFYFNDKNIQVEC-ENQILTVAVNKETEDGSSFSLQFPLS 4M5S Chain:A ((2-86)) -------------------------------------------MRLEKDRFSVNLDVKHFSPEELKVKVLGDVIEVHGKHEERQDEHGFISREFHRKYRIPADVDPLTITSSLSSDGVLTVNGPRKQV------------

General information: TITO was launched using: RESULT: Template: 4M5S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 222 -25036 -112.77 -333.81 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -112.77 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.791

(partial model without unconserved sides chains): PDB file : Tito_4M5S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4M5S-query.scw PDB file : Tito_Scwrl_4M5S.pdb: