Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIALIAHDKKKQDMVQFTTAYRDILKNHDLYATGTTGLKIHEATGLQIERFQSGPLGGDQQIGALIAANALDLVIFLRDPLTAQPHEPDVSALIRLCDVYSIPLATNMGTAEILVRTLDEGVFEFRDLLRGEEPNV 1S89 Chain:D ((14-129)) --IALVAHDHCKQMLMSWVERHQPLLEQHVLYATGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPNDPDVKALLRLATVWNIPVATNVATADFIIQS-------------------

General information: TITO was launched using: RESULT: Template: 1S89.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 555 -103889 -187.19 -895.59 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain D : 0.82 3D Compatibility (PKB) : -187.19 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.425

(partial model without unconserved sides chains): PDB file : Tito_1S89.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1S89-query.scw PDB file : Tito_Scwrl_1S89.pdb: