Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIRGILIAVLGIAIVGTGYWGYKEHQEKDAVLLHAENNYQRAFHELTYQVDQLHDKIGTTLAMNSQ------KSLSPALIDVWRITSEAHNSVSQLPLTLMPFNKTEELLSKIGDFSYKTSVRDLDQKPLDKNEYTSLNKLYQQSEDIQNELRHVQHLVMSKNLRWMDVEMALASDEKQSDNTIINSFKTVEKNVGAFSTGTDLGPSFTSTKKEEKGFSHLKGKQISEQEAKQIAERFAPDDNYSIKVVKSGKKTNRDVYS-ISMKDPD-HKAVIYMDITK-KGGHPVYLIQNREVKDQKISLNDGSNR---ALAFLKKNGFETDDLEIDESAQYDKIGVFSYVPVENKVRMYPEAIRMKVALDDGEVVGFSARDFLTSHRKRTIPKPAITEAEAKSKLNKNVQVRETR---LALITNELGQEVLCYEMLGTIENDTFRMYINAKDGSEEKVEKLKNAEPIYKDL-- 5EZ7 Chain:A ((2-392)) MPQALST---DILIVGGG-----------IAGLWLNARLRRAGYA-TVLVES--ASLGGGQSVKSQGIIHGGTGASEAIADMPRRWRACLGSDGELDL-----RGVRLLSEAHYLWSPVEQAKGEDLPPALRDK------------GFKGKAYRLTEIVF-------DVPDLIRRLAELAGDSLLAGERIEPLREGRELAGLCVD-----------------GREIRAQ-----------------RVVLSAGAGNEALLRELGLEQPAMQRRPLHMVMVKAATLKPLYAHCLGAGPKPRITVTTHPTRDGQSVWYLGGDIAETDGVARDEAAQIAE--------ARRELAKLLPWIDLGQA------------QWATLRVDRAEP------AQSNLLRPDNAFLAEQGRLLVGWPTKLALAPDFADRVCARLEEDGIR---PSEHAALPQLPRPPLAEPAWEVAFA

General information: TITO was launched using: RESULT: Template: 5EZ7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1965 102965 52.40 297.59 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 52.40 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.156

(partial model without unconserved sides chains): PDB file : Tito_5EZ7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EZ7-query.scw PDB file : Tito_Scwrl_5EZ7.pdb: