Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRD--MKKEECVRLISHFKFLSAMDIGERRKPQNGSLTLKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQYAKKHFNRNIVTLEDPVETRDEDVLQVQVN---EKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLHTRDAKGAIYRLLEFGIN------MNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYG-KNLQQCIQEAKGN--------H---ANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD 5FL3 Chain:A ((10-354)) -----QSLVEMLKAMVQARASDIHLQA---GAPPTVRIDGKLRPFGNRPLTPKEVEAIARALLTPEQLEELEYRKEMDFAYTI--P-GVARFRCNLLRQRGSFGLVMRVVSEVIP-SFEALGLPREVM---ESLAAKERGLILVTGPTGSGKSTTLAALIDHINLHYAKNIITIEDPIEFLHKHKKSLVVQREVGLDTDSFYTGLKYALRQDPDVIMVGEMRDRETVEAALMAAQTGHLVLSTLHTLDAWRTINRIIDFFPLHEHRQVRVLLAESLLGILSQRLLPKADG----------------QGRVLALEILIATPYVREL--LKDEEKTPQIKEAMMEGSLYGMRTFDQHLVELYTEGLISLEDALSAATSPH-

General information: TITO was launched using: RESULT: Template: 5FL3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1659 3948 2.38 12.26 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 2.38 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.449

(partial model without unconserved sides chains): PDB file : Tito_5FL3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FL3-query.scw PDB file : Tito_Scwrl_5FL3.pdb: