Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIIMKEAIIATHNPGKVKEFKEILEPRGYDVKSLA-EIGFTEEIEETGHTFEENAIMKAEAVAKAVNKMVIADDSGLSIDNLGGRPGVYSARYAGEQKDDQANIEKVLSELKGIEKEQRTARFRCALAVSIPGE-ETKTVEGHVEGYIAEEPRGEYGFGYDPIFIVKDKDKTMAELTSDEKNKISHRADALKKLSKLLEA 1VP2 Chain:A ((15-201)) ---KLTVYLATTNPHKVEEIKMIAPEW-MEILPSPEKI---EVVEDGE-TFLENSVKKAVVYGKKLKHPVMADDSGLVIYSLGGFPGVMSARFMEEH-SYKEKMRTILKMLEGK---DRRAAFVCSATFFDPVENTLISVEDRVEGRIANEIRGTGGFGYDPFFIPDGYDKTFGEIP-HLKEKISHRSKAFRKLFSVLEK

General information: TITO was launched using: RESULT: Template: 1VP2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 984 18943 19.25 102.39 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : 19.25 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.583

(partial model without unconserved sides chains): PDB file : Tito_1VP2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VP2-query.scw PDB file : Tito_Scwrl_1VP2.pdb: