Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVSIRRSFEAYVDDMNIITVLIPAEQKEIMTPPFRLETEITDFPLAVREEYSLEAKYKYVCVSDHPVTFGKIHCVRASSGHKTDLQIGAVIRTAAFDDEFYYD--G-ELGAVYTADHTVFKVWAPAATSAAVKLSHPNK-SGRTFQMTRL-EKGVYAVTVTGDLHGYEYLFCICNNS------EWMETVDQYAKAVTVNGEKGVVLRPDQM-----KWTA-PLKPF----SHPVDAVIYETHLRDFSIHENSGM-INKGKYLALTETDTQTANGSSSGLAYVKELGVTHVELLPVNDFAGVDEEKP-------------------------------------------------------------LDAYNWGYNPLHFFAPEGSYASNPHDPQTRKTELKQMINTLH-QHGLRVILDVVFNHVYKRENS-----PFEKTVPGYFFRHDEC-GMPSNGTGVGNDIASERRMARKFIADCVVYWLEEYNVDGFRFDLLGILDIDTVLYMKEKATKAKPGILLFGEGWDLATPLPHEQKAALANAPRMPGIGFFNDMFRDAVKGNT-FHLK-----ATGFALGNGESA---------------QAVMHGIAGSS------------------GWKA-LAPIVPEPSQSINYVESHDNHTFWDKMSFALP-QENDSRKRSRQRLAAAIILLAQGVPFIHSGQEFFRTKQGVENSYQSSDSINQLDWDRRET---------------------------------FKEDVHYIRRLISLRKAHPAFRLRSAADIQRHLECLTL----KEHLIAYRLYDLDE-----VDEWKDIIVIHHASPDSVEWRLPNDIPYRLLCDPSGFQEDP------TEIKKTVAVNGIGTVILYLASDLKSFA 2FHF Chain:A ((271-1072)) ----------------------------------------------------------------------------------ILSSATQVQTAGVLDDTYAAAAEALSYGAQLTDSGVTFRVWAPTAQQVELVIYSADKKVIASHPMTRDSASGAWSWQGGSDLKGAFYRYAMTVYHPQSRKVEQYEVTDPYAHSLSTNSEYSQVVDLNDSALKPEGWDGLTMPHAQKTKADLAKMTIHESHIRDLSAWDQTVPAELRGKYLALTAQES----NMVQHLKQLSASGVTHIELLPVFDLATVNEFSDKVADIQQPFSRLCEVNSAVKSSEFAGYCDSGSTVEEVLTQLKQNDSKDNPQVQALNTLVAQTDSYNWGYDPFHYTVPEGSYATDP-EGTARIKEFRTMIQAIKQDLGMNVIMDVVYNHTNAA-GPTDRTSVLDKIVPWYYQRLNETTGSVESAT-CCSDSAPEHRMFAKLIADSLAVWTTDYKIDGFRFDLMLYHPKAQILSAWERIKALNPDIYFFGEGWDSNQSD-RFEIASQINLK-GTGIGTFSDRLRDAVRGGGPFDSGDALRQNQGVGSGAGVLPNELTTLSDDQARHLADLTRLGMAGNLADFVLIDKDGAVKRGSEIDYNGAPGGYAADPTEVVNYVSKHDNQTLWDMISYKAAQEADLDTRVRMQAVSLATVMLGQGIAFDQQGSELLRSKSFTRDSYDSGDWFNRVDYSLQDNNYNVGMPRSSDDGSNYDIIARVKDAVATPGETELKQMTAFYQELTALRKSSPLFTLGDGATVMKRVDFRNTGADQQTGLLVMTIDDGMQAGASLDSRVDGIVVAINAAPESRTLQDFAGTSLQLSAIQQAAGDRSLASGVQVAADGSVTLPAWSVAVLELPQGE----

General information: TITO was launched using: RESULT: Template: 2FHF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3653 75811 20.75 121.69 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 20.75 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.443

(partial model without unconserved sides chains): PDB file : Tito_2FHF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FHF-query.scw PDB file : Tito_Scwrl_2FHF.pdb: