Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKHTFVLFLSLILLVLPGCSAEKSSADTAKKTLTIYSTMSTDSERDTFRKLAAAFEKEHSDIHVSLHF-P-GNDYENMMRVRMAANDLPDLFDTHG--WGKIRYGEYTADLRDMKWTQDLDPNLNSILKNKSGKVYAYPINQAKDGLAYNRNILDRYGIA-PPETMDDFIKALRTIKEKSKGSIVPFWFAGYDKSSFAQYYDQFATPLLITDPA--HNEKKQLINGTFQWS-KFTYLSEILKQMQKEKLINIDAVTAKKSQLIELMAQNKIAFTMQGGTLGQDVAQINPNVKVGIIPTPAIHPGDDPIWIGGERYTLAAWKD--SPQLKEAKDFIAFMARPANAKQMAEATSLPSGLTNVKA--DIFYANDYEYY-QDVKVEPY--FDRLYLPNGMWD-VLGTVGQELAADILAPQDISQKLGREYKRLREQSETQGAENNE 3QUF Chain:A ((27-411)) ---------------------------GGKTKISFYSYFK----DNQIGEVVKGFEKKNPDITLDVQYGQDPAQYISTLQTRLAGGKPPTIFNLTMDNRTDVMKSGAALDISGEDFLDGIDDTNFAL-FQQDGKTYGMPVSAWVGAFFYNKDILKKAGYDKFPKTWDEFIEMGKKIN-S-NG-STAFLEDFNTQIA-GS-FTGLLASY-YGEQGKSGDLDADIWSGKSTFTKDWTPVFKRWEAAAKAGVIPQKSVGLSADQVKQEFVSGNLGVMRSGPWDLPDLQKS--DIDFGVAPFPAYSKEDGQWINGGPDQGFAIASRASDKEKAAAKKFLAYLNSEEGLEAFTSAAGTLSLSSKYNAEPPAELK---DVVDNYFKQNKFYWVNWPKSPT-VMSTEGIAQQQKIVQGQISAKDAAKALDAKWATL-------------

General information: TITO was launched using: RESULT: Template: 3QUF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2033 99484 48.93 269.60 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 48.93 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.491

(partial model without unconserved sides chains): PDB file : Tito_3QUF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3QUF-query.scw PDB file : Tito_Scwrl_3QUF.pdb: