Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLEYGFKDDSLSLHTDLYQINMAETYWRDGIHEKKAIFELFFRRLPFENGYAVFAGLEKAI-EYLENFKFTDSDLSYLQDELGYHE----------DFIEYLRGLSFTGSLYSMKEGELVFNNEPIMRVEAPLVE----AQLIETALLNIVNYQTLIATKAARIKGVIGDEVALE-------------FGTRRAHEMDAAMWGARAALIGGFSATSNVRAGKRFNI------------PVSGTHAHALVQAYRDEYTAFKKYAETHKD--CVFLVDTYDTLRSGMPNAIRVAKE---FGDRINFIGIRLDSGDLAYLSKKARKMLDEA------------GFTDAKVIASSDLDEHTIMN----LKAQGARID--VWGVGTKLITAYDQPALGAVYKLVAIEEDGKMVDTIKIS-SNPEKVTTPGRKKVYRIINQSNHHSEGDYIALYDEQVNDQKRLRMFHPVHTFISKFVTNFYAKDLHELIFEKGILCYQNPEISDIQQYVQDNLSLLWEEYKRISKPEEYPVDLSEDCWSNKMQRIHEVKSRIEEELEEE 2E5D Chain:A ((16-491)) -------EFNILLATDSYKVTHYKQYPPN---TSKVYSYFECREKKY--EETVFYGLQYILNKYLKGKVVTKEKIQEAKDVYKEHFQDDVFNEKGWNYILEKYDGHLPIEIKAVPEGFVIPRGNVLFTVENTDPECYWLTNWIETILVQS-WYPITVATNSREQKKILAKYLLETSGNLDGLEYKLHDFGYRGVSSQETAGIGASAHLVN-FKGTDTVAGLALIKKYYGTKDPVPGYSVPAAEHSTITAWGKDHEKDAFEHIVTQFSSVPVSVVSDSYDIYNACEKIWGEDLRHLIVSRSTQAPLIIRPDSGNPLDTVLKVLEILGKKFPVTENSKGYKLLPPYLRVIQGDGVDINTLQEIVEGMKQKMWSIENIAFGSGGGLLQKLTRDLLNCSFKCSYVVTNGLGINVFKDPVADPNKRSKKGRLSLHR-------TPAGNFVTLEEGKGDLEEY-------------------GQDLLHTVFKNGKV-TKSYSFDEIRKNAQLN-----------------------------------------------

General information: TITO was launched using: RESULT: Template: 2E5D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2081 60975 29.30 151.68 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 29.30 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.383

(partial model without unconserved sides chains): PDB file : Tito_2E5D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2E5D-query.scw PDB file : Tito_Scwrl_2E5D.pdb: