Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEEKIYDVIIIGAGPAGMTAAVYTSRANLSTLMIERGIPGGQMANTEDVENYPGFESILGPELSNKMFEHAKKFGAEYAYGDIKEVID-GKEYKVVKAGSKEYKARAVIIAAGAEYKKIGVPGEKELGGRGVSYCAVCDGAFFKGKELVVVGGGDSAVEEGVYLTRFASKVTIVHRRDKLRAQSILQARAFDNEKVDFLWNKTVKEIHEENGKVGNVTLVDTVTGEESEFKTDGVFIYIGMLPLSKPFENLGI-TNEEGYIETNDRMETKVEGIFAAGDIREKSL--RQIVTATGDGSIAAQSVQHYVEELQETLKTLK 3F8R Chain:C ((16-318)) ------FDVIIVGLGPAAYGAALYSARYMLKTLVIGE-TPGGQLTEAGIVDDYLGLIEIQASDMIKVFNKHIEKYEVPVLL-DIVEKIEN--EFVVKTKRKGEFKADSVILGIGVKRRKLGVPGEQEFAGRGISYCSVCDAPLFKNRVVAVIGGGDSALEGAEILSSYSTKVYLIHRRDTFKAQPIYVETVKKKPNVEFVLNSVVKEIKGDK-VVKQVVVENLKTGEIKELNVNGVFIEIGFDPPTDFAKSNGIETDTNGYIKVDEWMRTSVPGVFAAGDCTSAWLGFRQVITAVAQGAVAATSAYRYVTE---------

General information: TITO was launched using: RESULT: Template: 3F8R.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1795 -61854 -34.46 -208.96 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain C : 0.81 3D Compatibility (PKB) : -34.46 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.488

(partial model without unconserved sides chains): PDB file : Tito_3F8R.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3F8R-query.scw PDB file : Tito_Scwrl_3F8R.pdb: