Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFQIGDNIVYPMHGAGIIEAIEEKEFLEEKQQYYVIRMSISNMTVMIPTSKILSSNIRPVTDILALKHIMHIFQHGE-SDRLLPWKQRYKINTDKIKTGEIQEGAE-VVRDLMRMKKEKALNASEKKMLDNAYEFLISELEVIKGITEKQIKSFG--------- 4L5G Chain:A ((3-164)) EFRPGDKVVLPPYGVGVVAGIAQRSVSGVSRAYYQVDFPGSRSKAYVPVEAPHSVGLRKALAPEEVPVILDLLKNGRMPLPK-QWAARHRKTSEILADGNPYRIAQMAGQ-LRAWEVERGLPDLDRQALRRAIHLLAEEVAQSLEITVQEAKRLFEEAWGEELN

General information: TITO was launched using: RESULT: Template: 4L5G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 568 -62641 -110.28 -414.84 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -110.28 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.488

(partial model without unconserved sides chains): PDB file : Tito_4L5G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4L5G-query.scw PDB file : Tito_Scwrl_4L5G.pdb: