Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTHPLFLEPVFKERLWGGTKLRDAFGYAIPSQKTGECWAVSAHAHGSSSVKNGPLAGKTLDQVWK-DHPE---------IFGFPDGKVFPLLVKLLDANMDLSVQVHPDDDYAKLHENGDLGKTECWYIIDCKDDAELILGHHASTKEEFKQRIESGDWNGLLRRIKIKPGDFFYVPSGTLH---ALCKGTLVLE-IQQ--NSDTTYRVYDYDRCNDQGQKRTLHIEKAMEVITIPHIDKVHTPEVKEVGNAEIIVYVQSDYFSVYKWKISGRAAFPSYQTYLLGSVLSGSGRIINNGIQYECNAGSHFILPAHFGEFTIEGTCEFMISHP 2Y0O Chain:A ((2-172)) -------------------------------------GITKEEVNSYYQKAGIVLTDEEVDQIQLMDYGLGKERKVGLQLFVYVNTDRYCSKELVLFPGQTCPEHRHP-------PVDGQEGKQETFRCRY--GKVYLYVEGEKTPLPKVLPPQEDREHYTVWHEIELEPGGQYTIPPNTKHWFQAGEEGAVVTEMSSTSTDKHDIFTDPRILEHHH------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2Y0O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 705 -27866 -39.53 -179.78 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -39.53 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.252

(partial model without unconserved sides chains): PDB file : Tito_2Y0O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Y0O-query.scw PDB file : Tito_Scwrl_2Y0O.pdb: