Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVNGIHHVSALTADAQKNLDFYKKVLGLKLVKKSVNQDE-PTMYHLFYGDEVANPGTELTFFEIPR-IAPFHAGTNSISSIGLRVPGTEALHYWKERFEEQQVTHSGISKRAGRDILAFQDHE-GQRLVLTADEEGKGYGLPVKQSGIPEEFSFRGLGPVELTVPYAEPTLHVLTNILGFTEISREPVEGQGTAVILESGE-GGAATEIHLIERNDLPRE--RQGKGSVHHVAFRVRDEEELAGWHRIISREGFSNS-GIVERYYFKALYFREPNGILFELSTDGPGFMVDENLDELGQTIALPPYLEHRRAEIEAKLKPIQ 4HUZ Chain:A ((4-315)) NHITSLHHITICTGTAQGDIDFFVKVMGQRFVKRTLFYDGSIPIYHLYFADELGTPGTVMTTFPTRRTGQKGRKGSNQFTVCTYAIP-KGSLEWWIGHLNAHGIATGEPGTRFGQRYVGFQHPDCGIDFEVLEDENDTRQ--PYDSPYVPIEHAQRGFHSWTASVRELEDMDFFMENCWNFEKIGEEG-----NRHRYRVKGTTESGTIIDLLHEPDRRQGSWTIAEGIIHHGAFAVPDMDIQARIKFETEGVGFTDFSDRKNRGYFESTYVRTPGGVMFEATH-SLGFTHDEDERSLGMDLKVSPQFDDKKHLIEQAMED--

General information: TITO was launched using: RESULT: Template: 4HUZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1754 -122016 -69.56 -400.05 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -69.56 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.486

(partial model without unconserved sides chains): PDB file : Tito_4HUZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HUZ-query.scw PDB file : Tito_Scwrl_4HUZ.pdb: