Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKYTLIATAPMGIEAVVAKEVRDLGY-E-C----K----VDNGKVIFEGD-------------ALAICRANLWLRTADRIKVQVASFKAKT---------FDELFEKTKAINWRSFIPENGKFPVIGKSVK-STLASVPDCQRIVKKAIVEKLKLQSGKANDWIEETGAEYKVEISLLKDQALITLDSSGT-GLHKRGYRVDQGGAPIKETLAAALVQLTNWTPDRPFVDPFCGSGTIAIEAALIGQNIAPGFNRDFVSEDWEWIGKDLWNKARLEVEEKANYDQPLTIFASDIDHRMVQIAKENAEEAGLGDLIQFKQMQVKDFTTNL-EFGVIVGNPPYGERLGEKKAVEQMYKEMGQAFEPL--DTWSVYMLTSNEN--FEEAYGRKATKKRKLFNGFIKTDYYQYWSKVRPQRKKTENA 3TM4 Chain:A ((1-366)) ---MKFLLTTAQGIEDIAKREVSLLLKKLGISFQIEEKPLGIEGRLLLEAEKAYYVDEKGRKRELSISTYLNENSRLLHRVIIEIASEKFNGIEKDESEEALKRIKDFVSSLPVEQFVKVSETFAVRSFRKGDHNITSI-DIARTVGEAIFERLSRF--GTP-LVNLDHPAVIFRAELIKDVFFLGIDTTGDSSLHKRPWRVYDHPAHLKASIANAMIELAEL-DGGSVLDPMCGSGTILIELALRRYS--------------------------------------GEIIGIEKYRKHLIGAEMNALAAGVLDKIKFIQGDATQLSQYVDSVDFAISNLPYG------SMIPDLYMKFFNELAKVLEKRGVFITTEKKAIEEAIAENGFEIIHHRVIGHGGLMVHLYVVKL------------

General information: TITO was launched using: RESULT: Template: 3TM4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1695 36367 21.46 113.29 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 21.46 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.461

(partial model without unconserved sides chains): PDB file : Tito_3TM4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TM4-query.scw PDB file : Tito_Scwrl_3TM4.pdb: