Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEPLKSHTGKAAVLNRINVDTDQIIPK-QFLK-RIERTGYGR-FAFFDWRYDANG---------EPNPEFELNQPVY---QGASILIAGENFGCGSSREHAPWALDDYGFKIIIAPSFADIFHQNCFKNGMLPIRMPYDNWKQLVGQYENQSLQMTVD----LENQ-----LIHDSEG--NQIS--FEVDPHWKEMLINGYDEISLTLLLEDEIKQFESQRSSWLQA 1C96 Chain:A ((551-742)) -----LEDLQILIKVKGKCTTDHISAAGPWLKFRGHLDNISNNLLIGAINIENRKANSVRNAVTQEFGPVPDTARYYKQHGIRWVVIGDENYGEGASREHSALEPRHLGGRAIITKSFARIHETNLKKQGLLPLTFADPADYNK---I-HPVDKLTIQGLKDFAPGKPLKCIIKHPNGTQETILLNHTFNETQIEWFRAGS--------------------------

General information: TITO was launched using: RESULT: Template: 1C96.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 847 21656 25.57 132.05 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 25.57 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.431

(partial model without unconserved sides chains): PDB file : Tito_1C96.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1C96-query.scw PDB file : Tito_Scwrl_1C96.pdb: