Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIISDKVRQILSYYESENPGVRANLTRILMHGKLGGTGKLVILPVDQGFEHGPIKSFEVNPHAYDPHYHFQLAIDSGVSAYAAPLGMIEAGASTYAGMLPHILKLNSSNSLHSKSLTSDQAVTASVKDALRLGCTAVGFTIYPGSAKCFDMIEEARKIIAEAKSYGLAVVLWSYPRGEGISKEGETAVDVIAYAAHIAALLGANIIKVKLPTNHLEKEKIEAGNIKSLSKRIEYVKKSCFAGKRIVVFSGG-ESKSVNDIYNEAKEIKQGGGNGSIIGRNTFQRKKEEALSMLKDIMDIYT 1W8S Chain:E ((3-238)) -----------------------NLTEKFLR-IFARRGKSIILAYDHGIEHGP-ADFMDNPDSADPEYILRLARDAGFDGVVFQRGIAE---KYYDGSVPLILKLNGKTTLYNGEPVS--VANCSVEEAVSLGASAVGYTIYPGSGFEWKMFEELARIKRDAVKFDLPLVVESFPRGGKVVN--ETAPEIVAYAARIALELGADAMKIKY-----------TGDPKTFSWAVK------VAGKVPVLMSGGPKTKTEEDFLKQVEGVLEAGALGIAVGRNVWQRRDALKFARALAELVYGG

General information: TITO was launched using: RESULT: Template: 1W8S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -142523 for 2084 contacts (-68.4/contact) + 2D Compatibility (PS) -26569 + (NN) -16269 + (LL) 3008 1D Compatibility (HY) -15200 + (ID) 4650 Total energy: -202203.0 ( -97.03 by residue) QMean score : 0.535

(partial model without unconserved sides chains): PDB file : Tito_1W8S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1W8S-query.scw PDB file : Tito_Scwrl_1W8S.pdb: