Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGITKEEVNSYYQKAGIVLTDEEVDQIQLMDYGLGKERKVGLQLFVYVNTDRYCSKELVLFPGQTCPEHRHPPVDGQEGKQETFRCRYGKVYLYVEGEKTPLPKVLPPQEDREHYTVWHEIELEPGGQYTIPPNTKHWFQAGE-EGAVVTEMSSTSTDKHDIFTDPRI 1V70 Chain:A ((18-104)) ------------------------------------------AKIPVFQSERMLYDLYALLPGQAQKVHVHE------GSDKVYYALEGEVVVRVGEEE---------------------ALLAPGMAAFAPAGAPHGVRNESASPALLLVVTAPR------------

General information: TITO was launched using: RESULT: Template: 1V70.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 417 -36504 -87.54 -424.47 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -87.54 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.560

(partial model without unconserved sides chains): PDB file : Tito_1V70.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1V70-query.scw PDB file : Tito_Scwrl_1V70.pdb: