Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIISYKCPNCGSDMAFDSETGSLSCSSCGRQDNIESLPKENIAARFSDDEAKEYQCKNCGAVLITEAETTATTCSFCGGAAILADRLSGHLAPAKVIPFTISKQEAEQAFRKWCKKGLLTPRGFMSADRIK---SITGMYIPFWMFDLNSEVQVRANCTRVHQYEEGDYICTETEHFEAFRDINLDYLKIPVDASEKMKDELMDKLEPYSYEELKDFQTAYLAGYIAEKYNYTDEELFPRAKEKISSYIDSYIHSTFSGYTSVNVRDKHIHTKNVNSFYVLLPVWMVSYDYERAEHIFAMNGQTGKVVGKPPISRGKVAAWFSGIAGGTFLALKLVSLMMGGGF 2MN4 Chain:A ((1-94)) ------------------------------------------------------------------------------------MTPEQREFLPEILAEIIANLDPTKILEELLRRGLLTPAELQEVLDLKTPEEQAKKLIDFILKLSPADVQARINVLRAHGYQALADKLNKYLTLE----------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2MN4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 233 -32596 -139.90 -358.20 target 2D structure prediction score : 0.34 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : -139.90 2D Compatibility (Sec. Struct. Predict.) : 0.34 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.197

(partial model without unconserved sides chains): PDB file : Tito_2MN4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2MN4-query.scw PDB file : Tito_Scwrl_2MN4.pdb: