Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence METFKSILHEAQRIVVLTGAGMSTESGIPDFRSAGGIWTEDASRMEAMSLDYFLSYPRLFWPKFKELFQMKMSGSFEPNEGHLLLAELE----KQGKQVDIFTQNIDGLHKKAGSRHVYELHGSIQTAACPACGA---RYDLP------------------HLLEREVPECTAAGNNGDICGTVLKTDVVLFGDAV--MHFDTLYEKLDQADLLLVIGTSLEVAPAR-FVPEDASLIPGMKKVIINLEPTYCDSLFDMVIHQKIGEFARSLGMKK 4HDA Chain:B ((13-258)) MADFRKFFAKAKHIVIISGAGVSAESGVPTFRGAGGYWRKWQAQDLATPLA-FAHNPSRVWEFYH--YRREVMGSKEPNAGHRAIAECETRLGKQGRRVVVITQNIDELHRKAGTKNLLEIHGSLFKTRCTSCGVVAENYKSPICPALSGKGAPEPGTQDASIPVEKLPRCEEAG-----CGGLLRPHVVWFGENLDPAILEEVDRELAHCDLCLVVGTSSVVYPAAMFAPQVAAR--GVPVAEFNTETTPATNRF-------------------

General information: TITO was launched using: RESULT: Template: 4HDA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1096 -98325 -89.71 -451.03 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -89.71 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.477

(partial model without unconserved sides chains): PDB file : Tito_4HDA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HDA-query.scw PDB file : Tito_Scwrl_4HDA.pdb: