Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGKFEGKIALVTGGTSGIGLATAQKFVNEGAYVYITGRRQNELDKAV---NQIGK-NVTGVQGDISKLEDLDKLYDIIKQEKGKLDILFANAGIGNFLPLGEITEEQVDRTFDINVKGTIFTVQKAL-SLFPDKVGSIIVTGSTAGSI-GNPAFSVYGASKAALRALVRNWILDLKGTEIRVNVVSPGGILTPAYDELFGDALEEVLENSRNTVPAGKVGTPEEVANAVSFLASDESSYLTGVELFVDGGLAQV 3PK0 Chain:C ((5-250)) MFDLQGRSVVVTGGTKGIGRGIATVFARAGANVAVAGRSTADIDACVADLDQLGSGKVIGVQTDVSDRAQCDALAGRAVEEFGGIDVVCANAGVFPDAPLATMTPEQLNGIFAVNVNGTFYAVQACLDALIASGSGRVVLTSSITGPITGYPGWSHYGATKAAQLGFMRTAAIELAPHKITVNAIMPGNIMT----EGLLENGEEYIASMARSIPAGALGTPEDIGHLAAFLATKEAGYITGQAIAVDGG----

General information: TITO was launched using: RESULT: Template: 3PK0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1376 -139085 -101.08 -579.52 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain C : 0.81 3D Compatibility (PKB) : -101.08 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.518

(partial model without unconserved sides chains): PDB file : Tito_3PK0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PK0-query.scw PDB file : Tito_Scwrl_3PK0.pdb: