Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKAVIHNGKAGLLGLSVQDVPSTKPGYGEVKVKLKSAGLNHRDLFLMKNKS----EQDPHMILGSDGAGIIEEIGEGVKNVTVQTEVVIFPTLNWDLTENVPPVPEILGGPSDGTLAEYVIIPSQNAIKKPAYLSWEEAGVLPLSALTAYRALFTKGQLKKGEHLLIPGIGSGVATYALFMAKAIGAT-VSVTSRSEEKRKKALKLGADYAFDSYS-NWDEQLQ----GKKIDVVLDSIGPA-LFSEYFRHVKPNGRIVSFGASSGDNLSFPVR-SLFFPQVNVLGTSMG-SGEEFQAMLAFIDKHKLRP--VIDRIY-PLEKACEAYKRMQEGRQFGNIGIVME 2D8A Chain:A ((5-347)) MVAIMKTKPG--YGAELVEVDVPKPGPGEVLIKVLATSICGTDLHIYEWNEWAQSRIKPPQIMGHEVAGEVVEIGPGVEGIEVGDYVSVETHIVCGKCY-----TKIFGVDTDGVFAEYAVVPAQNIWKNPKSIPPEYATLQ-EPLGNAVDTVL--AGPISGKSVLITGA-GPLGLLGIAVAKASGAYPVIVSEPSDFRRELAKKVGADYVINPFEEDVVKEVMDITDGNGVDVFLEFSGAPKALEQGLQAVTPAGRVSLLGLYPGKV-TIDFNNLIIFKALTIYGITGRHLWETWYTVSRLLQSGKLNLDPIITHKYKGFDKYEEAFELMRAGK-TGKVVFML-

General information: TITO was launched using: RESULT: Template: 2D8A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1805 -690 -0.38 -2.19 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -0.38 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.516

(partial model without unconserved sides chains): PDB file : Tito_2D8A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2D8A-query.scw PDB file : Tito_Scwrl_2D8A.pdb: