Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGNTMCPKMESAFSLLGKRWNGLIIHVLMDGPKRFKEITETIPMISQKMLAERLKELEQNEIVERQVLPETPVKVIYTLTEKGTALQAVFQEMQAWADQFCEPGDTVCEEEK 2HZT Chain:A ((3-98)) -------LVEATLEVIGGKWK-VIL-HLTHGKKRTSELKRLMPNITQKMLTQQLRELEADGVINRIVYNQVPPKVEYELSEYGRSLEGILDML-AWGANHINR---------

General information: TITO was launched using: RESULT: Template: 2HZT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 269 -41440 -154.05 -445.59 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -154.05 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.652

(partial model without unconserved sides chains): PDB file : Tito_2HZT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HZT-query.scw PDB file : Tito_Scwrl_2HZT.pdb: