Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------MGRHKATIEGLV--MKERYYSHRAPGTERWITQPVCKVTRTEPIFEGYIDIEPIEIGGKVYIPGLNEYVIVTDRQRNIHNEWTYQTDRVIKTIIDEKSLKECEEHNNKKAKNNDTQNQRQIKTSWWQRLTKKD---------------------------------------------------------------------------------------------------------------------------------------------------------- 3MF2 Chain:A ((37-335)) ADPLDHLADKLFHSMGSDGVYARTALYESIVERLAALITSHREAGTEALRFPPVMSRAQLEKS--GYLKSFPNLLGCVCGLHGTEREINAAVSRFDAGGDWTTSLSPADLVLS-----PAACYPVYPIAASRGPLPKGGLRFDVAADCFRREPSKHLDRLQSFRMREYVCIGTPDDVSDFRERWMVRAQAIARDLGLTFRVDYASDPFFGRVGQMKAVSQKQQQLKFELLIPLRSEEQPTACMSFNYHREHFGTTWGIQDANGEPAHTGCVAFGMDRLAVAMFHTHGTDLSAWPAKVRDILGLQPH

General information: TITO was launched using: RESULT: Template: 3MF2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 259 5604 21.64 45.19 target 2D structure prediction score : 0.32 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : 21.64 2D Compatibility (Sec. Struct. Predict.) : 0.32 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.003

(partial model without unconserved sides chains): PDB file : Tito_3MF2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3MF2-query.scw PDB file : Tito_Scwrl_3MF2.pdb: