Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLKIDIKGRGKYKPASDYSIDDVKNVLMEKIFEESSRIINSDDDLEIIEKVDFRTDKINVLSLFSGCGGLDLGFELAGLAAVIGEQAAMEAFKDKDRFNELRNKSIFHTIYTNDLFKEANQTYKTNFPGHVIQHEKDIRQVKY--FPKCNLILGGFPCPGFSEAGPR-LIDDDRNFLYLHFIRSLIQAQPEIFVAENVKGMMTLGKGEVLNQIIEDFASAGYRVQFKLLNARDYGVPQLRERVIIEGVRKDISFN-YKYPSPTHGEETGLKPFKTLRDSIGDLVTDP-------GPYFTGSY------S--S--IY---MSRNRKKSWDEQSFTIQASGRQAPLHPGGLSMKKIGKDKWVFPDGEENHRRLSVKEIARVQTFPDWFQFSQGTNSQTSINNRLDKQYKQIGNAVPVLLAKAVASPIANWAINYLESSPNNKIKNRERKLSIRTFLRIKTS 5CIY Chain:A ((8-324)) ------------------------------------------------------QLTGLRFIDLFAGLGGFRLALESCGA----------------------------ECVYSNEWDKYAQEVYEMNFGE---KPEGDITQVNEKTIPDHDILCAGFPCQAFSISGKQKGFEDSRGTLFFDIARIVREKKPKVVFMENVKNFASHDNGNTLEVVKNTMNELDYSFHAKVLNALDYGIPQKRERIYMICFRNDLNIQNFQFPKPFE-------LNTFVKDLLLPDSEVEHLVIDRKDLVMTNQEIEQTTPKTVRLGIVGKGGQGERIYSTRGIAITLSAYGGGIFAKT-------------GGYLVNGKTRKLHPRECARVMGYPDSYKVH-PSTS---------QAYKQFGNSVVINVLQYIAYNIGSSLNF----------------------------

General information: TITO was launched using: RESULT: Template: 5CIY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1539 5276 3.43 18.01 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 3.43 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.426

(partial model without unconserved sides chains): PDB file : Tito_5CIY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5CIY-query.scw PDB file : Tito_Scwrl_5CIY.pdb: