Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNEIAEVLPESMKNALSEIPEQQWLEIEEVRIRINRPVELIRRGQPVYLSYAGTAEDAHLILSRLSNYSMYTLEEELKKGYVTIRGGHRVGLAGRVITENGGVKGLRDIASFNIRIARQKLGIAEPLLPYLYQN--SWLNTLIIGPPQTGKTTLLRDLARLSSTGKKNM--LPV------------KTGIVDERSEIAGCL--RGI----P-----QHQF---------GQ---------------RIDVLDACPKAEGLMMMIRS--MSPEVMIVDEIGRMEDTDA---LLEA---LHAGVSVIVSAHGWSISDLMKRPSLKRLWEERAFDRYLELSRAKG--PGTVSQIYDKDGNVLSRTTGVKTC 4Q9H Chain:A ((1047-1261)) -----------------------------------------------------------------------------------------------------------------------------PVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGSVFLDGKEIKQLNVQWLRAQLGIVSQEPILFDCSIAENIAYGDNSRVVSYEEIVRAAKEANIHQFIDSLPDKYNTRVGDKGTQLSGGQKQRI--AIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTI--------------QNADLIVVIQNGKVKEHGTHQQLLAQKG------------

General information: TITO was launched using: RESULT: Template: 4Q9H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 584 4102 7.02 26.63 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 7.02 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.318

(partial model without unconserved sides chains): PDB file : Tito_4Q9H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Q9H-query.scw PDB file : Tito_Scwrl_4Q9H.pdb: