Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKGMFFCARAVVPFMKKSKDGAIVNVGSIAGITGAGSSMPYAVSKSAVHGLTKSLAHALAPE-IRVSGVAPGAVATRWWAGRE----EKMKSMIGSLL-LQCIAEPDDVAKLICSLIEQES--LTGQIITIDSGQTL 4QEC Chain:B ((114-244)) --GTYFCMKYALKHMQKVSCGAIVNISSITGLSGFPYSILYGSTKHAVIGLTKGAAVEFADKGIKINAVAPGIIKTETLQ-KEIDSGEFSEDSISSIHPMQKLGTTLDVAKGIYFLANEDNNFITGHVLSIDGG---

General information: TITO was launched using: RESULT: Template: 4QEC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 409 -28145 -68.81 -228.82 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -68.81 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.305

(partial model without unconserved sides chains): PDB file : Tito_4QEC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QEC-query.scw PDB file : Tito_Scwrl_4QEC.pdb: