Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---MVLKRIRDMFVA--SVHEGLDKMENPKVMLNQYVRDME---SDIAKAKQTIVKQH---TIAYQFKKKYEEAAEVAGKRKNQAQLAFDAG-----EEELAKKALTEMKYLE---------------GKAAEHKASYEQANSQLADLKEQLAAL-----------ETKLQDVKDKKQALIARANAAKAKEHMNTTFDKIDSESA-YREFLRIENRIEEME-IRANYSKSAEA--GTELTRKEFADDV-------EAEIEKMRTLSLQKSDQTKAANE---------------------------------- 4P5W Chain:A ((31-394)) TKRADPAELRTIFLKYASIEKNGEFFMSPNDFVTRYLNIFSQPNPKTVELLSGVVDQTKDGLISFQEFVAFESVLCAPDALFMVAFQLFDKAGKGEVTFEDVKQVFGQTTIHQHIPFNWDSEFVQLHFGKERKRHLTYAEFTQFLLEIQLEHAKQAFVQRDNARTGRVTAIDFRDIMVTIRPHVLTPFVEECLVAAAGGTTSHQVSFSYFNGFNSLLNNMELIRKIYSTLAGTRKDVEVTKEEFVLAAQKFGQVTPMEVDILFQLADLYEPRGRMTLADIERIAPPNPDHVGGYKLAVATFAGIENKFGLYL

General information: TITO was launched using: RESULT: Template: 4P5W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 825 49263 59.71 218.94 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 59.71 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.393

(partial model without unconserved sides chains): PDB file : Tito_4P5W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4P5W-query.scw PDB file : Tito_Scwrl_4P5W.pdb: