Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLNLQGKTALVTGSTSGIGKAIASSLAEEGAAVIINGRREE-KVNQTIDELKTQHAEAVLYPAAFDLGTEEGCNELF----QAYPEVDILVNNLGIFEPAEYFDIPDDEWFRFFEVNIMSGVRLTRRYLHNMIEKKEGRVIFIASEAAIMPSQEMAHYSATKTMQLSISRSLAELATGTNVTVNTVMPGSTLTEGVETMLNSLYPGENLTVQEAEAR-FMKENRPTSIIQRLIRPEEIAHFVTFLSSPLSSAINGAALRADGGLVRSVF 2ZTL Chain:C ((2-256)) ----LKGKVAVVTGSTSGIGLGIATALAAQGADIVLNGFGDAAEIEKVRAGLAAQHGVKVLYDGA-DLSKGEAVRGLVDNAVRQMGRIDILVNNAGIQHTALIEDFPTEKWDAILALNLSAVFHGTAAALPHMKKQGFGRIINIASAHGLVASANKSAYVAAKHGVVGFTKVTALETAGQGITANAICPGWVRTPLVEKQISALAE-KNGVDQETAARELLSEKQPS---LQFVTPEQLGGTAVFLASDAAAQITGTTVSVDGG------

General information: TITO was launched using: RESULT: Template: 2ZTL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1339 -23795 -17.77 -95.56 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain C : 0.76 3D Compatibility (PKB) : -17.77 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.472

(partial model without unconserved sides chains): PDB file : Tito_2ZTL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZTL-query.scw PDB file : Tito_Scwrl_2ZTL.pdb: