Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------------------------------------------------------------------MGESTSLKEILSTLTKRIL--LIMIVTAAATAAGGLISFFALTPIYENSTQILVNQSKNERKEVQFNDVQTNLQLINTYNVIIK--SPAILDEVIKEMGLSMT-----SQELNDKITVSSEQD-----------------SQVVNISVRDENAETAAHIAN----------TIASVFQDKITSIMNVDNVSILSKAEVSEHPSPVSPKPLLNIAIAFAAGLAG---SIGLAFLLEHLDNTIKSE------EQLESLLDIPVLGTVSTIANEQKTAKTLQGFQSEKTGSGHFGA 4D0P Chain:A ((16-386)) NLYFQGGGRMAAAVRQDLAQLMNSSGSHKDLAGKYRQILEKAIQLSGAEQLEALKAFVEAMVNENVSLVISRQLLTDFCTHLPNLPDSTAKEIYHFTLEKIQPRVISFEEQVASIRQHLASIYEKEEDWRNAAQVLVGIPL-ETGQKQYNVDYKLETYLKIARLYLEDDDPVQAEAYINRASLLQNESTNEQLQIHYKVCYARVLDYRRKFIEAAQRYNELSYKTIVHESERLEALKHALHCTILASAGQQRSRMLATLFKDERCQQLAA--YGILEKMYLDRIIRGNQLQEFAAMLMPHQKATTADGSSILDRAVIEHNLLSASKLYNNITFEELGALLEIP-AAKAEKIASQMITEGRMNGFIDQIDGIVHFE-

General information: TITO was launched using: RESULT: Template: 4D0P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 986 25084 25.44 103.22 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 25.44 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.280

(partial model without unconserved sides chains): PDB file : Tito_4D0P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4D0P-query.scw PDB file : Tito_Scwrl_4D0P.pdb: