Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MERKTVLVIADLGGCPPHMFYKSAAEKYNLVSFIPRPFAITASHAALIEKYSVAVIKDKDYFKSLADFEHPDSIYWAHEDHDKPEEEVV--EEIVKVAGMFAVDAITTNNELFI--APMAKACERLGLR--GAGVQAAENARDKNKMRAAFNRAGVKSIKNK--RVTTLEDFRAALQEIGTPLILKPTYLASSIGVTLIKEMETAEAEFNRVNEYLKSINVPKAVTFEAPFIAEEFLQG-EYDDWYETSGYSDYISIEGIMA-DGEYFPVAIHDKTPQIGFTETSHITPS-ILDDDAKRKIVEAAKKANEGLGLENCATHTEIKLMK-NREAGLIESAARFAGWNMIPNIKKVFGVDMAQLLLDVLCFGKEADLPKGLLEQEPCYVADCHLYPQHFKENG--------QLPETAVDFVIESIDIPDGVLKGDTEIVSFSAAEAGTSVDLRLFEAFNSIAAFELKGSNSGDVAESIKQIQQQAKLTAKYALPV 2VPQ Chain:A ((2-413)) ---KKVLIANRG--EIAVRIIRACRDL-GIQ-----TVAIYSEGDKDALHT-----------------QIADEAYCVGPT--LSKDSYLNIPNILSIATSTGCDGVHPGYGFLAENADFAELCEACQLKFIGPSYQSIQKMGIKDVAKAEMIKANVPVVPGSDGLMKDVSEAKKIAKKIGYPVIIKATAGGGGKGIRVARDEKELETGFRMTEQEAQT-AFGN-----GGLYMEKFIENFRH------------IEIQIVGDSYGNVIHLGERDCTIQRRMQKLVEEAPSPILDDETRREMGNAAVRAAKAVNYE-NAGTIEFIYDLNDNKFYFMEMNTRIQVEH--PVTEMVTGIDLVKLQLQVAMGDVLPYKQEDI-----------KLTGHAIEFRINAENPYKNFM-PSPGKIEQY--LAPGG----Y-GVRIESACYTNYTIPPY-YDSMVAKLIIH--EPTRDEAIMAGIRALSEFVVL-------

General information: TITO was launched using: RESULT: Template: 2VPQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2290 40436 17.66 103.15 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 17.66 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.414

(partial model without unconserved sides chains): PDB file : Tito_2VPQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2VPQ-query.scw PDB file : Tito_Scwrl_2VPQ.pdb: