Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKFKWDEFFVTGDPLILGAQVSIALSTIAIIFVLTYFKKWKWLWSEWITTVDHKKLGIMYIISAVIMLFRGGVDGLMMRAQLALPNNSFLDSNHYNEIFTTHGTIMIIFMAMPFLI-GLINVVVPLQIGARDVAFPYLNNLSFWTFFVGAMLFNISFVIGGSP-NAGWTSYMPLASNDMSPGPGENYYLLGLQIAGIGTLMTGINFMVTILKMRTKGMTLMRMPMFTWTTLITMVIIVFAFPVLTVALALLSFDRLFGAHFFTLEAGGMPMLWANLFWIWGHPEVYIVILPAFGIFSEIISSFARKQLFGYKAMVGSIIAISVLSFLVWTHHFFTMGNSASVNSFFSITTMAISIPTGVKIFNWLFTMYKGRISFTTPMLWALAFIPNFVIGGVTGVMLAMAAADYQYHNTYFLVSHFHYVLIAGTVFACFAGFIFWYPKMFGHKLNERIGKWFFWIFMIGFNICFFPQYFLGLQGMPRRIYTYGPN-DGWTTLNFISTVGAFMMGVGFLILCYNIYYSFRYSTREISGDSWGVGRTLDWATSSAIPPHYNFA-VLPEVK-SQDAFLHMKEEKTELYPESKFKKIHMPSNSGRPFFMSVAFGLAGFG---LVFEWYWMGVVGLIGVLLCMVLRSFEYDNGYYISVDEIKETERKISE 2YEV Chain:A ((12-604)) ---------------------------------------WAVLW-DLLTTVDHKKIGLMYTATAFFAFALAGVFSLLIRTQLAVPNNQFLTGEQYNQILTLHGATMLFFFIIQAGLTGFGNFVVPLMLGARDVALPRVNAFSYWAFLGAIVLALMSYFFPGGAPSVGWTFYYPFSA---QSESGVDFYLAAILLLGFSSLLGNANFVATIYNLRAQGMSLWKMPIYVWSVFAASVLNLFSLAGLTAATLLVLLERKIGLSWFNPAVGGDPVLFQQFFWFYSHPTVYVMLLPYLGILAEVASTFARKPLFGYRQMVWAQMGIVVLGTMVWAHHMFTVGESTLFQIAFAFFTALIAVPTGVKLFNIIGTLWGGKLQMKTPLYWVLGFIFNFLLGGITGVMLSMTPLDYQFHDSYFVVAHFHNVLMAGSGFGAFAGLYYWWPKMTGRMYDERLGRLHFWLFLVGYLLTFLPQYALGYLGMPRRYYTYNADIAGWPELNLLSTIGAYILGLGGLVWIYTMWKSLRSGPK-APDNPWG-GYTLEWLTASPP-KAHNFDVKLPTEFPSERPLYDWKKKGVE-LKPEDPAHIHLPNSSFWPFYSAATLFAFFVAVAALPVPNVW--MWVFLALFAYGLVRWALEDEYSH---------------

General information: TITO was launched using: RESULT: Template: 2YEV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3209 -502578 -156.62 -859.11 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -156.62 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.368

(partial model without unconserved sides chains): PDB file : Tito_2YEV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YEV-query.scw PDB file : Tito_Scwrl_2YEV.pdb: