Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEQQVKDDIQRVFQLQKKQQKALRASTAEQRREKLQRFLDSVIAHEEEIIEAIRKDVRKPYHEVKKAEIEGTKKAIRDNMNNLEQWMAPKEVGSSLSPDANGILMYEPKGVTLILGPWNYPFMLTMAPLAASLAAGNSAIVKLSDFTMNTSNIAAKVIRDA-FDEKEVAIFEGEVEVATELLDQPFD--HIFFTGSTNVGKIVMTAAAK-HLASVTLELGGKSPTIIDSEYDLMDAAKKIAVGKFVNAGQTCIAPDYLFIKKDVQDRFAGILQTVVNAG--FMEDDHTPDRSKFTQIVNDRNFNRVKDLFDDAIERGAEV-VFGGVFDASDRTISPTVLKNVTPDMKIMQEEIFASILPMMNYEDIDEVIDYVNDRDKPLALYVFSKNQDLIDNVLQHTTSGNAAINDVVVHFSDVNLPFGGVNTSGIGSYHGVYGFKEFSHEKGVFIQAAE 5AC0 Chain:B ((158-498)) ----------------------------------------------------------------------------------------------------------EPVGVCGQIIPWNFPLLMFLWKIGPALSCGNTVVVKPAEQTPLTALHMGSLIKEAGFPPGVVNIVPGYGPTAGAAISSHMDVDKVAFTGSTEVGKLIKEAAGKSNLKRVSLELGGKSPCIVFADADLDNAVEFAHQGVFYHQGQCCIAASRLFVEESIYDEF--VRRSVERAKKYVLGNPLTPGVSQGPQI-DKEQYEKILDLIESGKKEGAKLECGGGPWGNKGYFIQPTVFSDVTDDMRIAKEEIFGPVQQIMKFKSLDDVIKRANNTFYGLSAGIFTNDIDKAITVSSALQSGTVWVNCYSV--VSAQCPFGGFKMSGNGRELGEYGFHEYTEVKTVTIKISQ

General information: TITO was launched using: RESULT: Template: 5AC0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1899 6394 3.37 19.14 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.73 3D Compatibility (PKB) : 3.37 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.485

(partial model without unconserved sides chains): PDB file : Tito_5AC0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5AC0-query.scw PDB file : Tito_Scwrl_5AC0.pdb: