Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKFDISVLSVAPLR-QG--------ETMKQGIDSAVSLAKAVDNMGYKRIWFAEHHNHD------AYASAATVSIVQHILANTKDIRVGSGGIMLPNHSPLIVAEQFGTLETLYPNRVDLALGRAPGTDQKTADVIRR---S-NHNGVFFFEREVNDILRFVGDK---------------------S-----VQGEV---RAYPGI-GTHVPVFVLGSSTDSAEIAAKLGLPYAFGAQFSPHSMEEALSIYRENFQPSSY-LQEPYVIACINVIAAESIDEASFISASHLQVY-------IDIYTNNLSKLIPPTENFLESLSQ--F-ELEILHSR--LGYTIMGDRETIRRELIDFQQMYHADELIVLSNIYELSKEIQSYEILKQVVDELFKKRMEQL 1TVL Chain:A ((4-410)) ADFIQFGAMIHGVGGTTDGWRHPDVDPSASTNIEFYMKKAQTAEKGLFSFIFIADGLFISEKSIPHFLNRFEPITILSALASVTKNIGLVG-TFSTSFTEPFTISRQLMSLDHISGGRAGWNLVTSP--QEGAARNHSKSNLPEHTERYEIAQEHLDVVRGLWNSWEHDAFIHNKKTGQFFDQAKLHRLNHKGKYFQVEGPLNIGRSKQGEPVVFQAGSSETGRQFAAKNADAIFTHS-NSLEETKAFYADVKSRAADEGRDPSSVRIFPGISPIVADTEEEAEKKYREFAELIPIENAVT--------------------EAKARNLTLREVAQEMAFPRTLFIGTPERVASLIETWFNAEAADGFIVGSD------IPGTLDAFVEKVIPILQER----

General information: TITO was launched using: RESULT: Template: 1TVL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1572 -171948 -109.38 -563.76 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -109.38 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.412

(partial model without unconserved sides chains): PDB file : Tito_1TVL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TVL-query.scw PDB file : Tito_Scwrl_1TVL.pdb: