Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQSQPVLTIVVPCFNEEEVFQETSHQLTEVVDDLIEEKLIAEDSKILFVDDGSKDRTWALIAMESIRNKKVTGLKLAC------------NVG-------HQKALLAGLHKAKNRSDCVISIDADLQDDISVI---RDFMLKYHEG--CEIVYGVRRSRKTDTFFKRTTALGFYRLMNKLGIKLIYNHADFRLMNKRSLEELE-RYPEANLFLRGIVPMIGFKSAEVLYDRKERFAGKTKYPLKKMLSFAFNGITSFSVAPIRFFTLLGFVLFFLSAVAGIGAFIQKLLGHTNAGWASLIISIWFLGGLQLMGIGIIGEYIGTIFSEVKRRPKYAIDIDLYNEQLSPLQRQEKERLEKKYS 1FO8 Chain:A ((1-196)) ----LAVIPILVIACDRST-VRRCLDKLLHYRPS-------AELFPIIVSQDCGHEETAQVIASYGS---AVTHIRQPDLSNIAVQPDHRKFQGYYKIARHYRWALGQIFHNFN--YPAAVVVEDDLEVAPDFFEYFQATYPLLKADPSLWCVSAWNDNGK-EQMVD---SSK----PEL-LYRTDFFPGLGWLLLAELWAELEPKWPK---------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1FO8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 633 -99287 -156.85 -628.40 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -156.85 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.419

(partial model without unconserved sides chains): PDB file : Tito_1FO8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FO8-query.scw PDB file : Tito_Scwrl_1FO8.pdb: