Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLTKLLTYLTTEPSVNDSQDPEITSIEMDSREVKKGSLFVCVKGYTVDGHDFAQKAVENGAAAIVAEREVDVNVPVIIVRQSLRALSVLSDAFYGQPTKKLQLIGITGTNGKTSTTHMVDEILKKAGKRTGLIGTMYM-------KIGDE-TLPV-----------------------KNTTPESVTLQKT--FKKMNDKHVDTAIMEVSSHAL-SLGRVHGCDYDIAVFTNLTQDHLDYHK-TMDEYRHAKSLLFSQLG-GAFNHEHPKRAVLNADDEASAYFEKV-T-AAHISTYGIKNDADVMAKNISITAQGTSFDLVTNKGTKH-ITMSLVGQFNVYNVLAAVATCIAA---GI-PFEIITEAVEELHGVRGRFELVNQQQEFPVIVDYAHTPDSLENVLETCRDM-TEGKLFVVVGCGGDRDKTKRPKMAKIAVELADEPIFTS-DNPRSEDPRAILRDMEAGVENAYYHSIANREQAIFFAIANA-----------KKGDVVLIAGKGHETYQQIGNETFDFDDAEVAARAIVELNKNKTNS 2VOS Chain:A ((57-472)) ----------------------------------------------------------------------------------------------LGSPQRSYPSIHIAGTNGKTSVARMVDALVTALHRRTGRTTSPHLQSPVERISIDGKPISPAQYVATYREIEPLVALIDQQSQAS--PAMSKFEVLTAMAFAAFADAPVDVAVVEVGMGGRWDA--TNVINAPVAVITPISIDHVDYLGADIAGIAGEKAGIITRAPSPD------TVAVIGRQVPKVMEVLLAESVRADASVARE--DSEFAVLRRQIAVGGQVLQLQGLGGVYSDIYLPLHGEHQAHNAVLALASVEAFFGAQLDG-DAVRAGFAAV-TSPGRLERMRS--APTVFIDAAHNPAGASALAQTLAHEFDFRFLVGVLSVLGDKDV---DGILAALEPVFDSVVVTHNGSPRALDVEALALAAGERFGPDRVRTAENLRDAIDVATSLVDDAAADPDVAG--RTGIVITGSV----------------------------------

General information: TITO was launched using: RESULT: Template: 2VOS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2077 -206237 -99.30 -597.79 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -99.30 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.483

(partial model without unconserved sides chains): PDB file : Tito_2VOS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2VOS-query.scw PDB file : Tito_Scwrl_2VOS.pdb: