Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKALIYARVSTNKEQQETSLKRQEEELTAIAAENGMEVVKVISEKASGYEMDRDGVFELLDEIKNADIDVILVQDETRLGRGNAKIALLHCIYREGVKVYTTAHRGELELSEADSMVLEIVSIVEEYQRKIHNMKIRRGMKRAVKNGFKPQKNLKNQHGNSGKEKIEVPISEIVRLRANKLTFAEIAATLRGFGYDVSKATVHRRFQEYIENEETAE 3LHK Chain:A ((62-201)) AKIIGYARVSFNAQK--DDLERQIQLIKSYAEENGWDI-QILKDIGSGLNEKRKNYKKLLKMVMNRKVEKVIIAYPDRLTRFGFET-LKEFFKSYGTEIVIINKK--HK-TPQEELVEDLITIVSHFAGKLYGMHSHKYKKLTKTVK----------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3LHK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 549 -38903 -70.86 -277.88 target 2D structure prediction score : 0.78 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -70.86 2D Compatibility (Sec. Struct. Predict.) : 0.78 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.456

(partial model without unconserved sides chains): PDB file : Tito_3LHK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3LHK-query.scw PDB file : Tito_Scwrl_3LHK.pdb: