Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLRIGSHVSMSGKHM-LLAASQEAVSYGANTFMIYTGAPQNTRRKKIEDLNIEAGRAHMQENGIDEI--IVHAPYIINIGN-----TTNPSTFELGVDFLRSEIERTAAIGAKQIVLHPGAHVG-AGAEAGIKKIIEGLNEVID--PNQNVQIALETMAGKGSE--CGRSFEELAQIIEGVTHNEQLSVCFDTCHTHDAGYNI--VEDFDGVLNEFDKIIGIDRIKVLHINDSKNVK--GARKDRHENIGFGEIGFDALQYVVHHEQLKDIPKILETPYVGEDKKNKKPPYRFEIEMLKEKQFDDTLLEKILQQ 1QTW Chain:A ((1-285)) MKYIGAHVSAAG---GLANAAIRAAEIDATAFALFTKNQRQWRAAPLTTQTIDEFKAACEKYHYTSAQILPHDSY------LINLGHPVTEALEKSRDAFIDEMQRCEQLGLSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQ--GVTAVIENTAGQ--GSNLGFKFEHLAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARTVGFKYLRGMHLNDAK-STFGSRVDR-HHSLGEGNIGHDAFRWIMQDDRFDGIPLILETI----NPDI----WAEEIAWLKAQQTE-----KAVA-

General information: TITO was launched using: RESULT: Template: 1QTW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1521 22523 14.81 84.04 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 14.81 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.573

(partial model without unconserved sides chains): PDB file : Tito_1QTW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1QTW-query.scw PDB file : Tito_Scwrl_1QTW.pdb: