Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEPLKSHTGKAAVLNRINVDTDQIIPK----------QFLK---------------RIERTGYGRFAFFDWRYDAN-----G--------EPNPEFELNQPVYQ---GASILIAGENFGCGSSREHAPWALDDYGFKIIIAPSFADIFHQNCFKNGMLPIRMPYDNWKQLVGQYENQSLQMTVDLE-----NQLIHD--SEGNQISF--EV-DPHWKEMLINGYDEISLTLLLEDEIKQFESQRSSWLQA 2B3Y Chain:A ((661-877)) -----IVDAYVLLNLGDSVTTDHISPAGNIARNSPAARYLTNRGLTPREFNSYGSRRGNDAVMARGTFANIRLLNRFLNKQAPQTIHLPSGEILDVFDAAERYQQAGLPLIVLAGKEYGAGSSRDWAAKGPFLLGIKAVLAESYERIHRSNLVGMGVIPLEYLPGENADALGL--TGQERYTIIIPENLKPQMKVQVKLDTGKTFQAVMRFDTDVELTYFLNGG--------------------------

General information: TITO was launched using: RESULT: Template: 2B3Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 883 6995 7.92 42.14 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 7.92 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.389

(partial model without unconserved sides chains): PDB file : Tito_2B3Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2B3Y-query.scw PDB file : Tito_Scwrl_2B3Y.pdb: