Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVDEMVLITQQWLNDTYSGK--HGYNPVEESGKTGWDTIYGLTRALQIELGISEPADNFGPTTQRLF---KPLKRQAPDSKPSNMNFILQGALWCKGFNPGGFTGVFYEKT---ENAVKEFQKAAGLTTQDG---------IVTTLIMKALL-------DMSAFKLVSGGDSRIRQIQQNLNRDYNDY----IGLMPCDGLYG------------RDTNKALIYALQKEEGMSTSVANGFFGNGTTSLCPTLTPGDSRTGFVLIVQYALYCNGKSFDPG-----EFDGKYGVGVVSAVKA-----FQEFMCLPQTGYADM-------PTIKALLSSSGDTTRTASAC---DTATIIT----------------AEKAQTLRNNGYKTVGRYLTGNVRTSSGL---------TSKALTSKELAVILD--------AGLKVFPI--YQDGGYESSYFVKDQGTRDAY------------------SAASAARRLGFPSGTTIYFAVDFDAY--DYEVTDKIIPYFQEIKSAFTKMQTFSTAPKYEIGVYGPRN--------ICIRTSEAGLTKYSFVANMSTGFSGNLGYPMPNNWAFDQFYEGTIGSGSGSIGIDKD------GYSGRDSGASNVNPPSDPVYDARLRTLTDILSTIPALENLTSLANAMFEFDTTETIFTSPELDIILSTSLLAT---IPSEGSPNTITITNG-KPGAYITGLLGDTQTSLTASQIDSYQNLLNSLSLSVRNGYLEVYVN---------PTAESLNIQIKIYTPDIPVGDN--------VTTGLTTTITFKIKTY-----------KGVPVTSPESELALDWPSYDQYLFPVVGVAALLLIGNMGSDLTNNKGVKVATALSAMLLAIFAYYTS 4QOY Chain:C ((5-886)) PNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEARKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASK-KDLELGGHMASFQSSATIYDVCFNHFFRAR--NEQDGGDLVYFQGHISPGVYARAFLEGRLTQEQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLKDTSKQTVYAFLGDGEMDEPESKGAITIATR---------EKLDNLVFVINCNLQRLDGPVTGNGKIINELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYVREHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTIKGYGMGDAAIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHTYLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLKNKSIKDRLVPIIADEARTFGM-----EGLFRQIGIYSPNGKEDEKGQILQEGINELGAG-CSWLAAA-TSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFLIGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAY----AYEVAVIMHDGLERMYGEKQENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVREAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPAVASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVV----VAALGELAKRGE--IDKKVVADAIAKFNIDADKVNPRLA

General information: TITO was launched using: RESULT: Template: 4QOY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 3325 -178968 -53.82 -254.58 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain C : 0.66 3D Compatibility (PKB) : -53.82 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.162

(partial model without unconserved sides chains): PDB file : Tito_4QOY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QOY-query.scw PDB file : Tito_Scwrl_4QOY.pdb: