Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIHLSILDQAPVSK-G--------ESPVTTLQHSVELAQLSEQWGYKRYWFAEHHSTK------GLASTAPEIMIARIAAQTNTIRVGSGGVLLPQYSPFKVAETFRQLEALYPNRIDLGVGRSPGGTTKTRLALTD----GVKKSLTEFNRQLQDVSYFLTDS---------------------L-PPDHPYA---GIKAAPLI-GTAPELWVLGLGENSARRAAHQGIGYVFGHFINPER-GENAFRIYRESFRPSAHF-SNPSALFTIFVICAKTDEEAEELALSQDLWL-------LRVGKGLDSRVPSIEEAKAHPY-----TASDKKLIEE-N-RKRMVIGSPTTVKQQLLDLTGCYETNEIMVLCNVFDFEAKKESYERLAELFL 1TVL Chain:A ((7-404)) -IQFGAMIHGVGGTTDGWRHPDVDPSASTNIEFYMKKAQTAEKGLFSFIFIADGLFISEKSIPHFLNRFEPITILSALASVTKNIGLVGTF-STSFTEPFTISRQLMSLDHISGGRAGWNLVTSPQE--GAARNHSKSNLPEHTERYEIAQEHLDVVRGLWNSWEHDAFIHNKKTGQFFDQAKLHRLNHKGKYFQVEGPLNIGRSKQGEPVVFQAGSSETGRQFAAKNADAIFTHS-NSLEETK-AFYADVKSRAADEGRDPSSVRIFPGISPIVADTEEEAEKKYREFAELIPIENAVT----------------------EAKARNLTLREVAQEMAFPRTLFIGTPERVASLIETWFNAEAADGFIVGSD------IPGTLDAFVEKVI

General information: TITO was launched using: RESULT: Template: 1TVL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1517 -133306 -87.87 -448.84 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -87.87 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.411

(partial model without unconserved sides chains): PDB file : Tito_1TVL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TVL-query.scw PDB file : Tito_Scwrl_1TVL.pdb: