Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFIHIVGPGDSLFSIGRRYGASVDQIRGVNGLDETNIVPGQALLIPLYVYTVQPRDTLTAIAAKAFVPLERLRAANPGISPNALQAGAKITIPSISNYIAGTLSFYVLRNPDLDRELINDYAPYSSSISIF-EYHIAPNGDIA-NQL--NDAAAIETTWQRRVTPLATITNLTS--GGFSTEIVHQVLNNPTARTNLVNNIYDLVSTRGYGGVTIDFEQVSAADRDLFTGFLRQLRDRLQAGGYVLTIAVPAKTSDNIP-WLRGYDYGGIGAVVNYMFIMAYDWHHAG-S--------EPGPVAPITEIRRTIEFTIAQVPSRKIIIGVPLYGYDWIIPYQPGTVASAISNQNAIERAMRYQAPIQYSAEYQSPFFRYS-----------DQQGRTHEVWFEDVRSMSRKMQIVREYRLQAIGAWQLTLGFTPGPWLLRKFFTIRKV 4Q6T Chain:A ((4-340)) --------------------------------------------------------------------------------------------------APFVLAYTD-GQVEASYSNLQAFHRNLSAVGLGSTYGLTVTGKLRQDGMNETTQNIIRFAKSQSLPLYPTVSDYNEDIGAFDPAISHSILNDRALSAGTVKQLVKLAKEGGFAGINLDFEKVEPRNRAAFCAFVKTLGNALHASNKKLIISIPPKLSDTEPEYLQGYDYKALGAAVDYFQVMTYDQVGPGWSSGGFHNEAWPGPESGFDWQQALLSYAVSRVPASKVLAGLPTYGQDYSIGNR----VHWSAYQEIIAE---HRAAIHRDAASATPYATWGPVKSFVDGVEWTPERAQPVLWYDDAASIKTKTALVTRLGLGGTSVWAMGYENAGFWAALQSGLK----

General information: TITO was launched using: RESULT: Template: 4Q6T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1826 -203003 -111.17 -654.85 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -111.17 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.444

(partial model without unconserved sides chains): PDB file : Tito_4Q6T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Q6T-query.scw PDB file : Tito_Scwrl_4Q6T.pdb: