Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----MASEMIVDHRQKAFELLKVDAEKILKLIRVQMDNLTMPQCPLYEEVLDTQMFGLSREIDFAVRLGLVDEKDGKDLLYTL-ERELSALHDAFTAK- 2CCQ Chain:A ((1-99)) GSASPAVAELCQNTPETFLEASKLLLTYADNILRNPNDEKYRSIRIGNTAFSTRLLPVRGAVECLFEMGFEEGETHLIFPKKASVEQLQKIRDLIAIER

General information: TITO was launched using: RESULT: Template: 2CCQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 355 -54025 -152.18 -580.91 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -152.18 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.394

(partial model without unconserved sides chains): PDB file : Tito_2CCQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CCQ-query.scw PDB file : Tito_Scwrl_2CCQ.pdb: